https://richiekohman.com richie kohman Fri, 12 Jul 2019 03:09:03 +0000 https://richiekohman.com en https://richiekohman.com/Home Thu, 27 Dec 2012 21:01:50 +0000 richie kohman https://richiekohman.com/Home Richie E. Kohman, Ph.D I am an applied scientist whose mission is to develop and utilize innovate approaches to solve pressing challenges, primarily in neuroscience.&nbsp; Previosuly I was CSO of the Wyss Center for Bio and Neuroengineering where I led the Center’s scientific activities to better understand the brain’s structure and function as well as to develop improved diagnostics and therapeutics for brain disorders.&nbsp; Previously I led the Synthetic Biology Platform Harvard’s Wyss Insitute at Harvard University along with Faculty Lead George Church.&nbsp; I have more than fifteen years of professional experience in academic, industrial, and nonprofit research settings, and have overseen research projects in a myriad of area suchs as neurotechnology, gene therapy, regenerative medicine, biological tissue mapping, stem cell engineering, and nanotechnology. This is my personal website which follows my career progress. &nbsp; Additional profiles can be found at Google Scholar, LinkedIn, and ResearchGate as well as my personal Twitter account. https://richiekohman.com/Background Wed, 15 May 2019 02:01:01 +0000 richie kohman https://richiekohman.com/Background Chief Scientific Officer2021-2023Wyss Center for Bio and NeuroengineeringGeneva, Switzerland Platform Lead - Synthetic Biology Platform2018-2021 Advanced Technology Team Research Scientist2016-2018Wyss Institute at HarvardBoston, MALab affiliation- George ChurchBiomaterials Engineer2015-2016Expansion Technologies Inc.Cambridge, MAAffiliate2013-2021Massachusetts Institute of TechnologyMcGovern Institute for Brain Research and MIT Media Lab Lab affiliation - Ed BoydenCollaborator2011-2015Harvard Medical School, Massachusetts Eye and Ear InfirmaryDepartment of Otolaryngology-Head and Neck Surgery Lab affiliation - Ben BleierPostdoctoral Fellow2010-2015Boston UniversityDepartment of Biomedical Engineering Lab affiliation - Xue HanGraduate Research Assistant2003-2009University of Illinois Urbana-Champaign Department of Chemistry and Department of Chemical and Biomolecular Engineering Lab affiliation - Steven Zimmerman and Hyunjoon KongEducationPhD Chemistry - University of Illinois Urbana-Champaign 2003-2009BS Chemistry - Santa Clara University 1999-2003Contactrekohman@gmail.com https://richiekohman.com/Publications Wed, 15 May 2019 02:01:07 +0000 richie kohman https://richiekohman.com/Publications 36) Article <img width="1473" height="559" width_o="1473" height_o="559" data-src="https://freight.cargo.site/t/original/i/d1e37e054eb7db4a640aef130e727cb31489e1c222215d3135759c77fc1218ff/ovarian.jpg" data-mid="169782943" border="0" data-scale="25" src="https://freight.cargo.site/w/1000/i/d1e37e054eb7db4a640aef130e727cb31489e1c222215d3135759c77fc1218ff/ovarian.jpg" /> Merrick Pierson Smela, Christian Kramme, Patrick Fortuna, Jessica Adams, Rui Su, Edward Dong, Mutsumi Kobayashi, Garyk Brixi, Venkata Srikar Kavirayuni, Emma Tysinger, Richie. E. Kohman, Toshi Shioda, Pranam Chatterjee, George M. Church. “Directed Differentiation of Human iPSCs to Functional Ovarian Granulosa-Like Cells via Transcription Factor Overexpression”&nbsp;eLife 2023;11:e83291. DOI: https://doi.org/10.7554/eLife.83291 Link 35) Comment Insoo Hyun, JC Scharf-Deering, Sarah Sullivan, John Dennis Aach, Paola Arlotta, Matthew L Baum, George M Church, Aaron Goldenberg, Henry T Greely, Parastoo Khoshakhlagh, Richie E Kohman, Melissa Lopes, Caroline Lowenthal, Aric Lu, Alex HM Ng, Sergiu P Pasca, Bruna Paulsen, Martina Pigoni, Christopher T Scott, David A Silbersweig, Mark A Skylar-Scott, Robert D Truog, Jeantine E Lunshof. “How Collaboration Between Bioethicists and Neuroscientists Can Advance Research”&nbsp;Nature Neuroscience, 2022, 25, 1399–1401. https://doi.org/10.1038/s41593-022-01187-2 Link 34) Preprint <img width="691" height="584" width_o="691" height_o="584" data-src="https://freight.cargo.site/t/original/i/eb1026a15ed462f2f9a05a91032af99eb2f5dfd64886ec45360c9abf37665098/Picture2.jpg" data-mid="169783534" border="0" data-scale="26" src="https://freight.cargo.site/w/691/i/eb1026a15ed462f2f9a05a91032af99eb2f5dfd64886ec45360c9abf37665098/Picture2.jpg" />Daniel R. Goodwin, Alex Vaughan, Daniel Leible, Shahar Alon, Gilbert L. Henry, Anne Cheng, Xiaoyin Chen, Ruihan Zhang, Andrew G. Xue, Asmamaw T. Wassie, Anubhav Sinha, Yosuke Bando, Atsushi Kajita, Adam H. Marblestone, Anthony M. Zador, Edward S. Boyden, George M. Church, Richie E. Kohman. “Expansion Sequencing of RNA Barcoded Neurons in the Mammalian Brain: Progress and Implications for Molecularly Annotated Connectomics” bioRxiv, 2022, 2022.07.31.502046. Link 33) Preprint Christian Kramme, Merrrick Pierson Smela, Bennett Wolf, Patrick R Fortuna, Garyk Brixi, Kalyan Palepu, Edward Dong, Jessica L Adams, Suhaas Bhat, Sabrina Koseki, Emma Tysinger, Teodora Stan, Richie E Kohman, Songlei Liu, Mutsumi Kobayashi, Toshi Shioda, George M Church, Pranam Chatterjee. “Efficient Human Germ Cell Specification from Stem Cells via Combinatorial Expression of Transcription Factors” bioRxiv, 2022, 2022.07.11.499564. Link 32) Article <img width="1977" height="542" width_o="1977" height_o="542" data-src="https://freight.cargo.site/t/original/i/9a9d05b2c435049a00735e95581f7d275061ec22a805a63c37b0feafb029efc0/feed.jpg" data-mid="169821293" border="0" data-scale="40" src="https://freight.cargo.site/w/1000/i/9a9d05b2c435049a00735e95581f7d275061ec22a805a63c37b0feafb029efc0/feed.jpg" />Mutsumi Kobayashi, Misato Kobayashi, Junko Odajima, Keiko Shioda, Young Sun Hwang, Kotaro Sasaki, Pranam Chatterjee, Christian Kramme, Richie E Kohman, George M Church, Amanda R Loehr, Robert S Weiss, Harald Jüppner, Joanna J Gell, Ching C Lau, Toshi Shioda. “Expanding homogeneous culture of human primordial germ cell-like cells maintaining germline features without serum or feeder layers” Stem Cell Reports, 2022, 17, 507-521. Link 31) Article <img width="1325" height="1325" width_o="1325" height_o="1325" data-src="https://freight.cargo.site/t/original/i/647b7014c44a5fea9432fcbbe5213fb75309673691dd523b09105be8a8063ed9/mega.jpg" data-mid="169821156" border="0" data-scale="27" src="https://freight.cargo.site/w/1000/i/647b7014c44a5fea9432fcbbe5213fb75309673691dd523b09105be8a8063ed9/mega.jpg" /> Christian Kramme, Alexandru M Plesa, Helen H Wang, Bennett Wolf, Merrick Pierson Smela, Xiaoge Guo, Richie E Kohman, Pranam Chatterjee, George M Church. “An integrated pipeline for mammalian genetic screening” Cell Reports Methods, STAR Protocols, 2021, 2, 100907. Link 30) Article <img width="1744" height="1268" width_o="1744" height_o="1268" data-src="https://freight.cargo.site/t/original/i/7b073c23b92f7a14b75d18df70143ce208d1ab53947c1230c24db0a9d835c616/stamps.jpg" data-mid="169821075" border="0" data-scale="29" src="https://freight.cargo.site/w/1000/i/7b073c23b92f7a14b75d18df70143ce208d1ab53947c1230c24db0a9d835c616/stamps.jpg" /> Christian Kramme, Alexandru M Plesa, Helen H Wang, Bennett Wolf, Merrick Pierson Smela, Xiaoge Guo, Richie E Kohman, Pranam Chatterjee, George M Church. “An integrated pipeline for mammalian genetic screening” Cell Reports Methods, 2021, 1, 100082. Link 29) Article <img width="706" height="569" width_o="706" height_o="569" data-src="https://freight.cargo.site/t/original/i/e4f125a6c26e3cccf1cb5c78dfdaedf271c8363e203da8876002b94de442693c/spike.jpg" data-mid="169820988" border="0" data-scale="19" src="https://freight.cargo.site/w/706/i/e4f125a6c26e3cccf1cb5c78dfdaedf271c8363e203da8876002b94de442693c/spike.jpg" /> Tal Gilboa, Limor Cohen, Chi-An Cheng, Roey Lazarovits, Augusta Uwamanzu-Nna, Isaac Han, Kettner Griswold, Nick Barry, David B Thompson, Richie E Kohman, Ann E Woolley, Elizabeth W Karlson, David Walt. “A SARS‐CoV‐2 Neutralization Assay using Single Molecule Arrays“ Angew. Chem. Int. Ed. Angew. Chem. Int. Ed. 2021, 60(49), 25966-25972. Link 28) Article <img width="827" height="1399" width_o="827" height_o="1399" data-src="https://freight.cargo.site/t/original/i/fd1d091387f726f8ebc8a85b2f77841b9bbea07cf3ed1d27c1ddcc85e78c9c72/bolaramis.jpg" data-mid="169815243" border="0" data-scale="17" src="https://freight.cargo.site/w/827/i/fd1d091387f726f8ebc8a85b2f77841b9bbea07cf3ed1d27c1ddcc85e78c9c72/bolaramis.jpg" />Songlei Liu, Sukanya Punthambaker, Eswar P R Iyer, Thomas Ferrante, Daniel Goodwin, Daniel Fürth, Andrew C Pawlowski, Kunal Jindal, Jenny M Tam, Lauren Mifflin, Shahar Alon, Anubhav Sinha, Asmamaw T Wassie, Fei Chen, Anne Cheng, Valerie Willocq, Katharina Meyer, King-Hwa Ling, Conor K Camplisson, Richie E Kohman, John Aach, Je Hyuk Lee, Bruce A Yankner, Edward S Boyden, George M Church. “Barcoded Oligonucleotides Ligated on RNA Amplified for Multiplex and Parallel in-situ Analyses” Nucleic Acids Research, gkab120, https://doi.org/10.1093/nar/gkab120Link 27) Article <img width="1280" height="1274" width_o="1280" height_o="1274" data-src="https://freight.cargo.site/t/original/i/09e7cade137435d6cbc722d95eb7735e5bfb6ee13fc23e375b8eb76ccb391518/F1.large.jpg" data-mid="97176128" border="0" data-scale="25" src="https://freight.cargo.site/w/1000/i/09e7cade137435d6cbc722d95eb7735e5bfb6ee13fc23e375b8eb76ccb391518/F1.large.jpg" /> Shahar Alon, Daniel R. Goodwin, Anubhav Sinha, Asmamaw T. Wassie, Fei Chen, Evan R. Daugharthy, Yosuke Bando, Atsushi Kajita, Andrew G. Xue, Karl Marrett, Robert Prior, Yi Cui, Andrew C. Payne, Chun-Chen Yao, Ho-Jun Suk, Ru Wang, Chih-Chieh (Jay) Yu, Paul Tillberg, Paul Reginato, Nikita Pak, Songlei Liu, Sukanya Punthambaker, Eswar P. R. Iyer, Richie E. Kohman, Jeremy A. Miller, Ed S. Lein, Ana Lako, Nicole Cullen, Scott Rodig, Karla Helvie, Daniel L. Abravanel, Nikhil Wagle, Bruce E. Johnson, Johanna Klughammer, Michal Slyper, Julia Waldman, Judit Jané-Valbuena, Orit Rozenblatt-Rosen, Aviv Regev, IMAXT Consortium, George M. Church, Adam H. Marblestone, Edward S. Boyden “ Expansion Sequencing: Spatially Precise in situ Transcriptomics in Intact Biological Systems ” Science, 2021, Vol. 371, Issue 6528, eaax2656 DOI: 10.1126/science.aax2656. Link 26) Article <img width="813" height="393" width_o="813" height_o="393" data-src="https://freight.cargo.site/t/original/i/98809d5c945c13443379acf5130f249c32f7940229d66ea2d8bc00e9420f11ee/fig.jpg" data-mid="91523549" border="0" data-scale="28" src="https://freight.cargo.site/w/813/i/98809d5c945c13443379acf5130f249c32f7940229d66ea2d8bc00e9420f11ee/fig.jpg" />Alex H. M. Ng, Parastoo Khoshakhlagh, Jesus Eduardo Rojo Arias, Giovanni Pasquini, Kai Wang, Anka Swiersy, Seth L. Shipman, Evan Appleton, Kiavash Kiaee, Richie E. Kohman, Andyna Vernet, Matthew Dysart, Kathleen Leeper, Wren Saylor, Jeremy Y. Huang, Amanda Graveline, Jussi Taipale, David E. Hill, Marc Vidal, Juan M. Melero-Martin, Volker Busskamp, George M. Church “A Comprehensive Library of Human Transcription Factors for Cell Fate Engineering“ Nature Biotechnology, 2020,&nbsp;https://doi.org/10.1038/s41587-020-0742-6. Link 25) Perspective <img width="1424" height="806" width_o="1424" height_o="806" data-src="https://freight.cargo.site/t/original/i/f1fd5875177d8fb809e85bf89feef442c820c125d0adfc6b4b2a3c23f34baf9c/fig.jpg" data-mid="91523664" border="0" data-scale="30" src="https://freight.cargo.site/w/1000/i/f1fd5875177d8fb809e85bf89feef442c820c125d0adfc6b4b2a3c23f34baf9c/fig.jpg" />Brian M Turczyk, Michele Busby, Allison L Martin, Evan R Daugharthy, Daniel Myung, Richard C Terry, Samuel A Inverso, Richie E Kohman, George M Church “Spatial Sequencing: A Perspective” Journal of Biomolecular Techniques, 2020, Jul; 31(2): 44–46. Link 24) Preprint <img width="4778" height="2226" width_o="4778" height_o="2226" data-src="https://freight.cargo.site/t/original/i/e8e578bbd412daa8fd655bbc0146d19ef67cfeab51634fbbe38ba7cc91758fe0/Figure2.jpg" data-mid="102999095" border="0" data-scale="33" src="https://freight.cargo.site/w/1000/i/e8e578bbd412daa8fd655bbc0146d19ef67cfeab51634fbbe38ba7cc91758fe0/Figure2.jpg" /> Richie E Kohman*, George M Church* “Fluorescent in situ sequencing of DNA barcoded antibodies” bioRxiv, 2020, 2020.04.27.060624. *co-correscponding authors Link 23) Article <img width="872" height="934" width_o="872" height_o="934" data-src="https://freight.cargo.site/t/original/i/cf03ec071e680a1b5a8a1b84328fa07bad0ee27176192d88bb3d94d0646f8a3e/Fig.png" data-mid="61593453" border="0" data-scale="25" src="https://freight.cargo.site/w/872/i/cf03ec071e680a1b5a8a1b84328fa07bad0ee27176192d88bb3d94d0646f8a3e/Fig.png" /> Howon Lee, Daniel J. Wiegand, Kettner Griswold, Sukanya Punthambaker, Honggu Chun, Richie E. Kohman*, George M. Church* “ Photon-directed Multiplexed Enzymatic DNA Synthesis for Molecular Digital Data Storage” Nature Communications, 2020, 11, 5246. https://doi.org/10.1038/s41467-020-18681-5. *co-correscponding authors Link 22) Patent Kettner John Frederick Griswold Jr., Richie E. Kohman, George M. Church, Jonathan Rittichier “Cleavable Nucleotide Analogs and Uses Thereof ” US20200080122A1, 2020. Link &nbsp;21) Preprint <img width="1951" height="501" width_o="1951" height_o="501" data-src="https://freight.cargo.site/t/original/i/112d3b09c877324cc0d294826346a1de77c3ab1378fe7c74a492eeb135bf9747/image.jpg" data-mid="102998341" border="0" data-scale="32" src="https://freight.cargo.site/w/1000/i/112d3b09c877324cc0d294826346a1de77c3ab1378fe7c74a492eeb135bf9747/image.jpg" /> Yu Wang, Yitian Zeng, Sinem K. Saka, Wenxin Xie, Isabel Goldaracena, Richie E. Kohman, Peng Yin, George M. Church. “Multiplexed in situ protein imaging using DNA barcoded antibodies with extended hybridization chain reactions” bioRxiv, 2020, 274456v3. Link 20) Patent Richie E. Kohman and George M. Church “Targeted expansion fluorescent in situ sequencing ” WO2018226930A1, 2018. Link 19) Review <img width="767" height="695" width_o="767" height_o="695" data-src="https://freight.cargo.site/t/original/i/aeb2bceea9ac2fa1b95d03146e9b92d32be6d653790c3c1090664642fd657e0a/TOC.png" data-mid="169822983" border="0" data-scale="13" src="https://freight.cargo.site/w/767/i/aeb2bceea9ac2fa1b95d03146e9b92d32be6d653790c3c1090664642fd657e0a/TOC.png" /> Richie E Kohman, Aditya M Kunjapur, Eriona Hysolli, Yu Wang, George M Church. “From Designing the Molecules of Life to Designing Life: Future Applications Derived from Advances in DNA Technologies” Angew. Chem. Int. Ed. 2018, 57, 4313-4328. Link 18) Patent Richie E. Kohman “Combining Modified Antibodies with Expansion Microscopy for in-situ, Spatially-resolved Proteomics” US Patent App. 15/592,221, 2018. Link 17)&nbsp;Patent Xue Han, Richie E. Kohman, Susue Cha “Light-stimulated release of cargo from oligonucleotides” WO2017151748A1, 2017. Link 16)&nbsp;Patent Richie E. Kohman “Combining Protein Barcoding with Expansion Microscopy for in-situ, Spatially-resolved Proteomics” US Patent App. 15/446,005, 2017. Link 15) Article <img width="469" height="295" width_o="469" height_o="295" data-src="https://freight.cargo.site/t/original/i/0991b3d30638f5b7d468c764f55ac37bfd78a71950641e53fde2e3b7eda1b335/abstract2.jpg" data-mid="42277240" border="0" src="https://freight.cargo.site/w/469/i/0991b3d30638f5b7d468c764f55ac37bfd78a71950641e53fde2e3b7eda1b335/abstract2.jpg" />Richie E. Kohman, Susie S. Cha, Heng-Ye Man, and Xue Han. “Light-Triggered Release of Bioactive Cargo from DNA Nanocages.” Nano. Letters 2016, 16, 2781–2785. Link Highlighted in Boston University Link Highlighted in ReliaWire Link 14) Article <img width="495" height="225" width_o="495" height_o="225" data-src="https://freight.cargo.site/t/original/i/0b2f74d5f47abfa7caf7696a0f16c9bff10de8beb025b1fcc00b0556abaa2c46/Drug-Delivery-across-Blood-Brain-Barrier.jpg" data-mid="42277241" border="0" src="https://freight.cargo.site/w/495/i/0b2f74d5f47abfa7caf7696a0f16c9bff10de8beb025b1fcc00b0556abaa2c46/Drug-Delivery-across-Blood-Brain-Barrier.jpg" />Benjamin S. Bleier*, Richie E Kohman*, Kevin Guerra, Angela L. Nocera, Armine H. Kocharyan, Shreshtha Ramanlal, and Xue Han. “Heterotopic Mucosal Grafting Enables the Delivery of Therapeutic Neuropeptides Across the Blood Brain Barrier.” Neurosurgery 2016, 78, 448-457.*co-first authors Link Highlighted in NeuroscienceNews Link Highlighted in KurzweilAI Link Highlighted in Parkinson's News Today Link 13) Article <img width="495" height="249" width_o="495" height_o="249" data-src="https://freight.cargo.site/t/original/i/c7f3eb811a376056d652fee33b50c98a3171c6e51703aeafba33c4766aa645e7/unnamed.jpg" data-mid="42277236" border="0" src="https://freight.cargo.site/w/495/i/c7f3eb811a376056d652fee33b50c98a3171c6e51703aeafba33c4766aa645e7/unnamed.jpg" />Richie E. Kohman, and Xue Han. "Light-Sensitization of DNA Nanostructures via Incorporation of Photo-Cleavable Spacers." Chem. Commun. 2015, 51, 5747-5750. Link 12) Book Chapter <img width="160" height="228" width_o="160" height_o="228" data-src="https://freight.cargo.site/t/original/i/9e4386eb8665de13ff399d7e816674484c3af7bea8cc0cd8dda0540796e866b5/book1.jpg" data-mid="42277233" border="0" src="https://freight.cargo.site/w/160/i/9e4386eb8665de13ff399d7e816674484c3af7bea8cc0cd8dda0540796e866b5/book1.jpg" />Hua-an Tseng, Richie E. Kohman, and Xue Han. “Chapter 9: Optogenetics and Electrophysiology” Basic Electrophysiological Methods. Oxford University Press. 2015. Link 11) Article <img width="381" height="224" width_o="381" height_o="224" data-src="https://freight.cargo.site/t/original/i/064f6f271f21deaba67e0b8ab41961c2bbc7336bfdb077e1d695376871fa094f/jove.jpg" data-mid="42277234" border="0" src="https://freight.cargo.site/w/381/i/064f6f271f21deaba67e0b8ab41961c2bbc7336bfdb077e1d695376871fa094f/jove.jpg" />Richie E. Kohman, Xue Han, and Benjamin S. Bleier. “Heterotopic Mucosal Engrafting Procedure for Direct Drug Delivery to the Brain in Mice.” J. Vis. Exp. 2014. Link 10) Book Chapter <img width="160" height="227" width_o="160" height_o="227" data-src="https://freight.cargo.site/t/original/i/2d34501f059701ab74b2a4f87adfeeae92aaf703738599915ad924fc8ac51d13/book2.jpg" data-mid="42277232" border="0" src="https://freight.cargo.site/w/160/i/2d34501f059701ab74b2a4f87adfeeae92aaf703738599915ad924fc8ac51d13/book2.jpg" />Richie E. Kohman, Hua-an Tseng, and Xue Han. “Chapter 14 Optogenetics” The OMICs: Applications in Neuroscience. Oxford University Press. 2014. Link 9) Article <img width="500" height="139" width_o="500" height_o="139" data-src="https://freight.cargo.site/t/original/i/d11c1eb1cd3be0c38ed5409137058e79e424e5a519612fa3821870fe72d01bf6/ja-2013-00636d_0003.gif" data-mid="42277235" border="0" src="https://freight.cargo.site/w/500/i/d11c1eb1cd3be0c38ed5409137058e79e424e5a519612fa3821870fe72d01bf6/ja-2013-00636d_0003.gif" />John J. Schmidt*, Jae Hyun Jeong*, Richie E. Kohman*, Andrew Zill, Ross DeVolder, ArtemShkumatov, Lawrence Schook, Steven C. Zimmerman, and Hyunjoon Kong. “Leukocyte-Mimicking Stem Cell Delivery via in situ Coating of Cells with a Bioactive Hyperbranched Polyglycerol.” J. Am. Chem. Soc. 2013, 135, 8770-8773.*co-first authors Link Highlighted as J. Am. Chem. Soc. cover June 19, 2013, Volume 135, Number 24 LinkHighlighted in “Spotlight on Recent JACS Publications” in J. Am. Chem. Soc.J. Am. Chem. Soc., 2013, 135, 8763. Link Highlighted in “Editor’s Choice” in ScienceScience, 2013, 340, 389. Highlighted in Chem. Eng. News: “Putting Step Cells In Their Place” April 25, 2013 Link 8) Article <img width="480" height="390" width_o="480" height_o="390" data-src="https://freight.cargo.site/t/original/i/387823f60005636332fa7a3331b8399631fae38c11f536129e35b6d97421ceb2/journal.pone.0061694.g001_640.JPG" data-mid="42277237" border="0" src="https://freight.cargo.site/w/480/i/387823f60005636332fa7a3331b8399631fae38c11f536129e35b6d97421ceb2/journal.pone.0061694.g001_640.JPG" />Benjamin S. Bleier, Richie E. Kohman, Rachel Feldman, Shreshtha Ramanlal, and Xue Han.“Permeabilization of the Blood-Brain Barrier via Mucosal Engrafting: Implications for Drug Deliveryto the Brain.” PLOS ONE 2013, 8, e61694. Link Highlighted in ScienceDaily Link Highlighted in KirzweilAI Link 7) Article <img width="378" height="136" width_o="378" height_o="136" data-src="https://freight.cargo.site/t/original/i/f54b0672da5016f8377e659702f04ade194afa2af0111a7f0795730b72cdd08e/GA4_o.gif" data-mid="42277231" border="0" src="https://freight.cargo.site/w/378/i/f54b0672da5016f8377e659702f04ade194afa2af0111a7f0795730b72cdd08e/GA4_o.gif" />Xiao-Xiang Zhang, Caroline M. LaManna, Richie E. Kohman, Thomas J. McIntosh, Xue Han, andMark W. Grinstaff. “Lipid mediated DNA and siRNA Transfection Efficiency Depends on PeptideHeadgroup.” Soft Matter 2013, 9, 4472-4479. Link 6) Article <img width="446" height="219" width_o="446" height_o="219" data-src="https://freight.cargo.site/t/original/i/a8f4e6df1e662aeda8ff19f9b0058beec481d24176ae6e32aba3b5118aea8d77/1-s2.0-S0142961210014602-sc1.jpg" data-mid="42277238" border="0" src="https://freight.cargo.site/w/446/i/a8f4e6df1e662aeda8ff19f9b0058beec481d24176ae6e32aba3b5118aea8d77/1-s2.0-S0142961210014602-sc1.jpg" />Youyun Liang, Tor W. Jensen, Edward J. Roy, Chaenyung Cha, Ross J. DeVolder, Richie E. Kohman, Bao Zhong Zhang, Kyle B. Textor, Lauretta A. Rund, Lawrence B. Schook, Yen Wah Tong, and Hyunjoon Kong. “Tuning the Non-equilibrium State of a Drug-encapsulated Poly(ethylene glycol) Hydrogel for Stem and Progenitor Cell Mobilization.” Biomaterials 2011, 32, 2004-2012. Link 5) Article <img width="492" height="293" width_o="492" height_o="293" data-src="https://freight.cargo.site/t/original/i/7b0e5fa3a5f2e1bad99387d63619d199e650b1c9eb6d106940c24ed52af2464d/Untitled.jpg" data-mid="42277239" border="0" src="https://freight.cargo.site/w/492/i/7b0e5fa3a5f2e1bad99387d63619d199e650b1c9eb6d106940c24ed52af2464d/Untitled.jpg" />Andrew Zill, Alexandra Rutz, Richie E. Kohman, Alaaldin M. Alkilany, Catherine J. Murphy, Hyunjoon Kong, and Steven C. Zimmerman. “Clickable Polyglycerol Hyperbranched Polymers and Their Application to Gold Nanoparticles and Acid-Labile Nanocarriers.” Chem. Commun. 2011, 47, 1279-1281. Link 4) Article <img width="306" height="189" width_o="306" height_o="189" data-src="https://freight.cargo.site/t/original/i/1d97e3498dfb4f73676421bdc319b70cb795ce7dd0a33117a165037c4553b518/GA3_o.gif" data-mid="42277230" border="0" src="https://freight.cargo.site/w/306/i/1d97e3498dfb4f73676421bdc319b70cb795ce7dd0a33117a165037c4553b518/GA3_o.gif" />Richie E. Kohman, Chaenyung Cha, Steven C. Zimmerman, and Hyunjoon Kong. “Tuning Hydrogel Properties and Function Using Substituent Effects.” Soft Mater 2010, 6, 2150-2152. Link 3) Article <img width="433" height="277" width_o="433" height_o="277" data-src="https://freight.cargo.site/t/original/i/00ebcf6681d7a1f40953e02922ce9953775c4c4dce5cb55c9f62125310017658/mcontent_o.jpg" data-mid="42277229" border="0" src="https://freight.cargo.site/w/433/i/00ebcf6681d7a1f40953e02922ce9953775c4c4dce5cb55c9f62125310017658/mcontent_o.jpg" />Chaenyung Cha, Richie E. Kohman, and Hyunjoon Kong. “Biodegradable Polymer Cross-linker: Independent Control of Stiffness, Toughness and Hydrogel Degradation Rate.” Adv. Funct. Mater. 2009, 19, 3056-3062. Link 2) Article <img width="376" height="134" width_o="376" height_o="134" data-src="https://freight.cargo.site/t/original/i/99e565ed57c807371db74d84f88ed541040f915bdb9b2acafccf14ccf8b8c104/GA_o.gif" data-mid="42277228" border="0" src="https://freight.cargo.site/w/376/i/99e565ed57c807371db74d84f88ed541040f915bdb9b2acafccf14ccf8b8c104/GA_o.gif" />Richie E. Kohman, and Steven C. Zimmerman. “Degradable Dendrimers Divergently Synthesized via Click Chemistry.” Chem. Commun. 2009, 794-796. Link 1) Article <img width="346" height="170" width_o="346" height_o="170" data-src="https://freight.cargo.site/t/original/i/c63bda8d0db593095273f39cc1fff37fcc12ae44b576243214184f04a2a78daa/mcontent_o.gif" data-mid="42277227" border="0" src="https://freight.cargo.site/w/346/i/c63bda8d0db593095273f39cc1fff37fcc12ae44b576243214184f04a2a78daa/mcontent_o.gif" />Amy M. Balija, Richie E. Kohman, and Steven C. Zimmerman. Substituted 1,3,5-Triazaadamantanes: Biocompatible and Degradable Building Blocks.” Angew. Chem. Int. Ed. 2008, 47, 8072-8084. Link https://richiekohman.com/News Fri, 12 Jul 2019 03:09:03 +0000 richie kohman https://richiekohman.com/News March 2023 Our Wyss Center press release has been put together showcase our new collaboration on spinal cord regeneration.&nbsp; Read about the project here. February 2023 CBS Boston mentioned our new work on fertility resarch on a recent television&nbsp;broadcast <img width="1083" height="609" width_o="1083" height_o="609" data-src="https://freight.cargo.site/t/original/i/363e9d24b490bc5defcf57be13f772136e1680176de3f7849cb227f0eae20e69/christian.jpg" data-mid="169782716" border="0" data-scale="40" src="https://freight.cargo.site/w/1000/i/363e9d24b490bc5defcf57be13f772136e1680176de3f7849cb227f0eae20e69/christian.jpg" /> February 2023 The Wyss Institute wrote a press release about our new work on fertility research and ovarian organoids. <img width="800" height="800" width_o="800" height_o="800" data-src="https://freight.cargo.site/t/original/i/1cfb0975cc5074c0524a032213fdf60e9ca32bbbe17abf4ab3dff0ed11d9f42f/ovaroid.jpg" data-mid="169738561" border="0" data-scale="42" src="https://freight.cargo.site/w/800/i/1cfb0975cc5074c0524a032213fdf60e9ca32bbbe17abf4ab3dff0ed11d9f42f/ovaroid.jpg" /> July 2022I was featured in a story from Laborjournal about DNA data storage. Read&nbsp;Bits and Bytes from DNA - New DNA Synthesis Methods and Data Storage. <img width="800" height="450" width_o="800" height_o="450" data-src="https://freight.cargo.site/t/original/i/c412154b1aba7cd2727b26cf6525d929d18104f6da6a05758aaeb5c38bbd61d9/269a.jpg" data-mid="148796277" border="0" data-scale="70" src="https://freight.cargo.site/w/800/i/c412154b1aba7cd2727b26cf6525d929d18104f6da6a05758aaeb5c38bbd61d9/269a.jpg" />Novermber 2021 I started my role as CSO at the Wyss Center for Bio and Neuroengineering in Geneva Switzerland! <img width="1000" height="561" width_o="1000" height_o="561" data-src="https://freight.cargo.site/t/original/i/481c444be2cbe3ecc88e3f67349b76fbdb251bcc90e79999e08182ba45a4ad49/1108x622.jpg" data-mid="123587436" border="0" data-scale="56" src="https://freight.cargo.site/w/1000/i/481c444be2cbe3ecc88e3f67349b76fbdb251bcc90e79999e08182ba45a4ad49/1108x622.jpg" /> September 2021 The Wyss Institute wrote a synopsis of our new STAMPscreen technology for mamallian genetic screening.&nbsp; Special emphasis given to the heroes of the work - Christian Kramme, Alex Plesa, and Pranam Chatterjee. <img width="800" height="535" width_o="800" height_o="535" data-src="https://freight.cargo.site/t/original/i/a7ac4dd485e561c0a6ee371960ecf61d4daad9a99a918d5c313ca00b188d3c2f/STAMPScreen-MegaGate-LR-and-Gateway-LR-1146.jpg" data-mid="121070113" border="0" data-scale="49" src="https://freight.cargo.site/w/800/i/a7ac4dd485e561c0a6ee371960ecf61d4daad9a99a918d5c313ca00b188d3c2f/STAMPScreen-MegaGate-LR-and-Gateway-LR-1146.jpg" />&nbsp; July 2021 I was featured in an article by NEO.LIFE on the data storage in DNA.&nbsp; Read it here.&nbsp;<img width="1896" height="781" width_o="1896" height_o="781" data-src="https://freight.cargo.site/t/original/i/b3430efaf3bcfa22a96170c8751277cec13be3b272eca25bdd96cf98e62724d4/dna-storage.jpg" data-mid="113688198" border="0" data-scale="52" src="https://freight.cargo.site/w/1000/i/b3430efaf3bcfa22a96170c8751277cec13be3b272eca25bdd96cf98e62724d4/dna-storage.jpg" /> June 2021 I helped write a piece in American Scientist discussing the ethics of cerebral organoids and the the importance of what we call them.&nbsp; Check out&nbsp;Minibrains: What's in a Name? <img width="1156" height="726" width_o="1156" height_o="726" data-src="https://freight.cargo.site/t/original/i/a1d94f90205c3540051c801389c69cad928a3ac382774f0f9d8202befc6294a8/2021-109-4-206-lopes-2-figcap.jpg" data-mid="111944199" border="0" data-scale="54" src="https://freight.cargo.site/w/1000/i/a1d94f90205c3540051c801389c69cad928a3ac382774f0f9d8202befc6294a8/2021-109-4-206-lopes-2-figcap.jpg" /> June 2021 I wrote a short piece dedicated to discussing synthetic biology signatures in brain tissue. -&nbsp;link <img width="2000" height="1200" width_o="2000" height_o="1200" data-src="https://freight.cargo.site/t/original/i/491299138f52cbdc30ae6e01ec05003d78378bd72144dbb224e46fcc1f5ac28e/dreamstime_xl_118253882-scaled.jpg" data-mid="111943525" border="0" data-scale="56" src="https://freight.cargo.site/w/1000/i/491299138f52cbdc30ae6e01ec05003d78378bd72144dbb224e46fcc1f5ac28e/dreamstime_xl_118253882-scaled.jpg" /> April 2021Stepped away from the FRO.&nbsp; I am happy that I was able to get it started - passing the baton now. April 2021 I wrote a short story for Open Acess Government about Next Generation Sequencing in biological tissues.&nbsp; Read it here.&nbsp; March 2021 Successfully pitched a new Focused Research Organization (FRO) now enititled E11 Bio dedicated to brain mapping to philanthropic investors.&nbsp; Recieved a “definite yes” to move forward!&nbsp; <img width="1913" height="1035" width_o="1913" height_o="1035" data-src="https://freight.cargo.site/t/original/i/0f5843465eb010895240743e680decef5678ca24cbac80ada0b7f6614960b677/screenshot-051721.jpg" data-mid="120923459" border="0" data-scale="42" src="https://freight.cargo.site/w/1000/i/0f5843465eb010895240743e680decef5678ca24cbac80ada0b7f6614960b677/screenshot-051721.jpg" /> March 2021 Nature wrote a Technology Feature on DNA data storage and discussed our work&nbsp; Read the feature&nbsp;here. <img width="1172" height="659" width_o="1172" height_o="659" data-src="https://freight.cargo.site/t/original/i/2cb48b7dc743aa6e4688a247b45bede2788746d09682312f4dba37c107f1f786/Nat.jpg" data-mid="101004649" border="0" data-scale="61" src="https://freight.cargo.site/w/1000/i/2cb48b7dc743aa6e4688a247b45bede2788746d09682312f4dba37c107f1f786/Nat.jpg" /> March 2021 Harvard Magazine interviewed me and the team about our work on enzymatic DNA data storage.&nbsp; Read it here. <img width="632" height="474" width_o="632" height_o="474" data-src="https://freight.cargo.site/t/original/i/cf469cd4ed91a8052448ba8955875a783d6beb2f899455ba54a0257a6f1a972c/HM.jpg" data-mid="101004160" border="0" data-scale="53" src="https://freight.cargo.site/w/632/i/cf469cd4ed91a8052448ba8955875a783d6beb2f899455ba54a0257a6f1a972c/HM.jpg" /> January 2021 Our work on Expansion Sequencing is highlighted by the McGovern Institute.&nbsp; Read about it here. <img width="2000" height="1333" width_o="2000" height_o="1333" data-src="https://freight.cargo.site/t/original/i/be4c0f6765b983417ef02bf737885dd51cd80da9c3c31b9d65ceeae03e568add/MIT-Expansion-Sequencing-01-press.jpg" data-mid="101050376" border="0" data-scale="45" src="https://freight.cargo.site/w/1000/i/be4c0f6765b983417ef02bf737885dd51cd80da9c3c31b9d65ceeae03e568add/MIT-Expansion-Sequencing-01-press.jpg" /> December 2020 Our paper on transcritpion factor induced stem cell differentiation was published in Nature Biotechnology.&nbsp; I am happy to have contributed to the project. Read a press release about it&nbsp;here. <img width="813" height="393" width_o="813" height_o="393" data-src="https://freight.cargo.site/t/original/i/d7adb4202b0713a55ab35c2a2d3bb8f322ec0f68e644099ec4bcacc51b930d5f/fig.jpg" data-mid="93908398" border="0" data-scale="52" src="https://freight.cargo.site/w/813/i/d7adb4202b0713a55ab35c2a2d3bb8f322ec0f68e644099ec4bcacc51b930d5f/fig.jpg" /> November 2020 Research Outlook showcased our work on in situ analysis of biological tissues with an emphasis on synthetic biology approaches to molecular connectomics of mammalian brains.&nbsp; Read the story here. <img width="873" height="295" width_o="873" height_o="295" data-src="https://freight.cargo.site/t/original/i/34c937f14e823d55f2ab26409547de75f173abe6d73eff3a9c185263eb296d56/brain-barcoding-image-v2.jpg" data-mid="90682146" border="0" src="https://freight.cargo.site/w/873/i/34c937f14e823d55f2ab26409547de75f173abe6d73eff3a9c185263eb296d56/brain-barcoding-image-v2.jpg" /> October 2020 Our work enzymatic DNA synthesis &amp; DNA data storage was covered by Forbes&nbsp; Read the story about it here. <img width="480" height="270" width_o="480" height_o="270" data-src="https://freight.cargo.site/t/original/i/bca791905fd68f8b4727b99e616c6674938446b40e034c8e852b38034473d70c/forbes.jpg" data-mid="101003142" border="0" src="https://freight.cargo.site/w/480/i/bca791905fd68f8b4727b99e616c6674938446b40e034c8e852b38034473d70c/forbes.jpg" /> October 2020 Work from the project I co-lead on enzymatic DNA synthesis &amp; DNA data storage got published in Nature Communications!&nbsp; Read the story about it here from The Harvard Gazette. <img width="900" height="600" width_o="900" height_o="600" data-src="https://freight.cargo.site/t/original/i/e476bec1664052465d861076b7bf0fd0b293aa6eb633306bb6e3d100a586f8b4/istock-2500-900x600.jpg" data-mid="93905263" border="0" data-scale="49" src="https://freight.cargo.site/w/900/i/e476bec1664052465d861076b7bf0fd0b293aa6eb633306bb6e3d100a586f8b4/istock-2500-900x600.jpg" /> June 2020 Biocompare ran a story on in situ sequencing and highlighted some of our contributions. June 2020 Wired Magazine featured my quarentine story in a recent piece they ran.&nbsp; Read the story here.<img width="1673" height="289" width_o="1673" height_o="289" data-src="https://freight.cargo.site/t/original/i/f953adfa5dafb13510d6f410dfea57b529321eee6b10b9f5ec2bc9415404175a/Untitled.jpg" data-mid="73999278" border="0" data-scale="75" src="https://freight.cargo.site/w/1000/i/f953adfa5dafb13510d6f410dfea57b529321eee6b10b9f5ec2bc9415404175a/Untitled.jpg" /> April 2020 Harvard Medical School summarized&nbsp; some of the COVID-related projects that we are working on.&nbsp;Click here to read the story. <img width="850" height="425" width_o="850" height_o="425" data-src="https://freight.cargo.site/t/original/i/f947f3e9fedc48223fdabc31f9b8c5ba24d7c58c2f11ad0db3b66e8050d74e8d/GettyImages-172189440-850px.jpg" data-mid="101050472" border="0" data-scale="37" src="https://freight.cargo.site/w/850/i/f947f3e9fedc48223fdabc31f9b8c5ba24d7c58c2f11ad0db3b66e8050d74e8d/GettyImages-172189440-850px.jpg" /> February 2020 The Bioscience magazine Laborjournal featured a story about our new light-stimulated DNA synthesis technology.&nbsp; Click here to read the story. <img src="https://www.laborjournal.de/edkacheln/1949.jpg"> December 2019 Lab Manager magazine featured me in their “Ask the Expert” segment related to&nbsp;Innovations in Next-Gen Sequencing.&nbsp; See the story here to read about our efforts with in situ sequencing.&nbsp; <img src="https://freight.cargo.site/w/640/i/60ef3342214fc1fce11ba7d28c529252cb6a2414e68570aec209c8537c27dae2/Dec19_ATE_640x360.jpg"> September 2019 Our brain connectivity project was featured in a story by Endpoints.&nbsp; Click here to read the story and hear what I have to say. <img width="1798" height="1035" width_o="1798" height_o="1035" data-src="https://freight.cargo.site/t/original/i/467ad15dbeac2e6a23555f29828ee3bd2611ee36b4dff7d93576d0e048c8465e/brain.jpg" data-mid="50908362" border="0" data-scale="52" src="https://freight.cargo.site/w/1000/i/467ad15dbeac2e6a23555f29828ee3bd2611ee36b4dff7d93576d0e048c8465e/brain.jpg" /> May 2019 I was recently interviewed for by Simulations.&nbsp; I discussed my personal interests and story as well as some of the research projects I am currently pursueing at the Wyss Institute. April 2019 Along with Church Lab postdoc Eriona Hysolli, I represented the SynBio Platform at the NOVA Education Wonders event as part of the Cambridge Science Festival.&nbsp; <img width="960" height="720" width_o="960" height_o="720" data-src="https://freight.cargo.site/t/original/i/273714e8ab1fe2990689001f942185a02b59a70ec075ecb7080ae5fdc6f4a98a/58381177_10156426060813519_1415023326954258432_n.jpg" data-mid="46681315" border="0" data-scale="32" src="https://freight.cargo.site/w/960/i/273714e8ab1fe2990689001f942185a02b59a70ec075ecb7080ae5fdc6f4a98a/58381177_10156426060813519_1415023326954258432_n.jpg" /><img width="2016" height="1512" width_o="2016" height_o="1512" data-src="https://freight.cargo.site/t/original/i/ac813e7c926f1430ab29f10691fe3a476ccf181afddd41efea86687d4072c05f/58689446_10156426060783519_856962558144282624_o.jpg" data-mid="46681343" border="0" data-scale="32" src="https://freight.cargo.site/w/1000/i/ac813e7c926f1430ab29f10691fe3a476ccf181afddd41efea86687d4072c05f/58689446_10156426060783519_856962558144282624_o.jpg" /><img width="2016" height="1512" width_o="2016" height_o="1512" data-src="https://freight.cargo.site/t/original/i/039b4fd1666497d72c34d49d125e3572660b2e46ed2ba999bf1fa27ba69a1348/58378612_10156426060793519_4036320306690785280_o.jpg" data-mid="46681344" border="0" data-scale="32" src="https://freight.cargo.site/w/1000/i/039b4fd1666497d72c34d49d125e3572660b2e46ed2ba999bf1fa27ba69a1348/58378612_10156426060793519_4036320306690785280_o.jpg" /> February 2019 Science Magazine featured a story on single-cell RNA sequencing in which I was featured.&nbsp; The story is here: link <img width="1241" height="767" width_o="1241" height_o="767" data-src="https://freight.cargo.site/t/original/i/f416b29589bc3500339479520c3d7e2c8b48a1627240ab5e086cf0ad92ce4478/rna-seq.jpg" data-mid="50512771" border="0" data-scale="61" src="https://freight.cargo.site/w/1000/i/f416b29589bc3500339479520c3d7e2c8b48a1627240ab5e086cf0ad92ce4478/rna-seq.jpg" /> November 2018 I was promoted to Lead of the Synthetic Biology Platform at the Wyss Institute.&nbsp; I now oversee all of the ongoing research projects as well as lead the staff towards developing new world-impacting technologies! November 2017 Endpoints featured a story on George Church in which I was featured talking about our FISSEQ technology.&nbsp; The story is here: link <img width="1053" height="763" width_o="1053" height_o="763" data-src="https://freight.cargo.site/t/original/i/f8f1ce4294272029baee897d1079efd595e96a69f7c3bc884656b4bb79f83533/FISSEQ.jpg" data-mid="50511847" border="0" data-scale="64" src="https://freight.cargo.site/w/1000/i/f8f1ce4294272029baee897d1079efd595e96a69f7c3bc884656b4bb79f83533/FISSEQ.jpg" /> September 2017 I was promoted to the Advanced Technology Team at the Wyss Insitute with the goal of working on applied technologies to help translate them to the outside world.July 2017 IARPA MICrONS enters Phase 2, with me as the Co-PI and program manager! May 2017 IEEE Spectrum featured a story on our MICrONS project including data that I collected myself.&nbsp; The story is here: link <img width="945" height="594" width_o="945" height_o="594" data-src="https://freight.cargo.site/t/original/i/d1c355d70004f45e2c3a9ea76b43736ba5f8b096b8b470e5b3ca316febf3f153/microns.jpg" data-mid="50512035" border="0" data-scale="71" src="https://freight.cargo.site/w/945/i/d1c355d70004f45e2c3a9ea76b43736ba5f8b096b8b470e5b3ca316febf3f153/microns.jpg" /> July 2016 I left my position at Expansion Technologies to start as Staff Scientist in the Synthetic Biologly Platform at the Harvard’s Wyss Institute.&nbsp; I will be working on the IARPA MICrONS project which utilizes in situ sequencing to map neural connections.